Breviscapine, a traditional Chinese medicine, alleviates myocardial ischaemia reperfusion injury in diabetic rats

J-H. Jia; K-P. Chen; S-X. Chen; K-Z. Liu; T-L. Fan; Y-C. Chen

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Document Details :

Title: Breviscapine, a traditional Chinese medicine, alleviates myocardial ischaemia reperfusion injury in diabetic rats
Author(s): J-H. Jia , K-P. Chen , S-X. Chen , K-Z. Liu , T-L. Fan , Y-C. Chen
Journal: Acta Cardiologica
Volume: 63 Issue: 6 Date: 2008
Pages: 757-762
DOI: 10.2143/AC.63.6.2033394

Abstract :
Objective – The aim of this study was to explore the effects of breviscapine on the expressions of intercellular adhesion molecule-1(ICAM-1),ATPase activities and oxidative stress in ischaemia-reperfused (I/R) myocardium of diabetic rats.
Methods — Sprague Dawley rats were randomly divided into two groups (a diabetic group and a non-diabetic group), and each group divided into two subgroups including a control group and a breviscapine group. Reperfusion surgery was carried out in all rats. The contents of malonaldehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in serum and myocardial tissues were measured. The activities of Na⁺-K⁺-ATPase, Mg²⁺-ATPase, Ca²⁺-ATPase in myocardial mitochondria were measured. The ICAM-1 protein expressions in myocardium were determined with the immunohistochemical method.
Results — The activities of Na⁺-K⁺-ATPase, Mg²⁺-ATPase, Ca²⁺-ATPase were significantly increased in diabetic rats in the breviscapine group compared with the control group. Compared with the non-diabetic control group, the contents of MDA in serum and myocardium were significantly increased in the diabetic control group. Breviscapine led to a reduction of the contents of MDA in the diabetic and non-diabetic group. Compared with the non-diabetic control group, the activities of SOD and GSH-P_X in the myocardium were significantly decreased in the diabetic control group. The activities of SOD and GSH-P_X in serum and myocardium were increased in the diabetic and nondiabetic group after breviscapine treatment. Compared with the non-diabetic control group, the ICAM-1 protein expressions were increased significantly in the diabetic control group. Breviscapine decreased the ICAM-1 protein expression in the diabetic and the non-diabetic group.
Conclusion — Breviscapine may have protective effects on myocardial ischaemia reperfusion injury of diabetic rats by scavenging oxygen free radicals, decreasing the expressions of ICAM-1 protein in myocardium and increasing the activities of Na⁺-K⁺-ATPase, Mg²⁺-ATPase,Ca²⁺-ATPase in myocardial mitochondria.