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Document Details : Title: Interferon-γ upregulates retinoic acid-inducible gene-I in human pericardial mesothelial cells Author(s): M. Hatakeyama , T. Imaizumi , F. Terasaki , F. Mori , K. Tanji , F. Sato , H. Kijima , H. Suma , K. Wakabayashi , H. Yoshida , I. Fukuda , K. Satoh Journal: Acta Cardiologica Volume: 62 Issue: 6 Date: 2007 Pages: 553-557 DOI: 10.2143/AC.62.6.2024013 Abstract : Objectives — Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH box family proteins and serves as an intracellular signalling molecule involved in the regulation of various genes. Mesothelial cells are active in generating a variety of bioactive factors, and RIG-I may serve as a potential intracellular molecule regulating part of these factors.We have addressed the expression of RIG-I in pericardial mesothelial cells. Methods and results — Cultures of human mesothelial cells separated during cardiac surgery were established. Interferon (IFN)-γ was found to stimulate the expression, in cultured mesothelial cells, of RIG-I mRNA and protein in time- and concentration-dependent manners.We also performed immunohistochemical analysis of heart tissues to examine the expression of RIG-I in mesothelial cells. Intense immunoreactivity was detected in reactive pericardial mesothelial cells in non-specific pericarditis and in dilated cardiomyopathy. Conclusion — RIG-I may be involved in the inflammatory reaction in pericardial mesothelial cells. |
