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Document Details : Title: Total flavones from Elsholtzia blanda reduce infarct size and improve heart function during acute myocardial infarction by inhibiting myocardial apoptosis in canines Author(s): LING, Haiyun , LOU, Yija , WU, Honghai , LOU, Honggang Journal: Acta Cardiologica Volume: 60 Issue: 3 Date: June 2005 Pages: 295-301 DOI: 10.2143/AC.60.3.2005007 Abstract : Objective — The current study tests the hypothesis that inhibition of apoptotic cell death with total flavones from Elsholtzia blanda (TFEB), a traditional Chinese medicine, reduces infarct size and improves heart function during myocardial ischaemia induced by coronary occlusion in canines. Methods and results — Myocardial apoptosis was detected by the TdT-mediated dUTP nick end labelling (TUNEL) method and DNA laddering. Infarct size of the left ventricle, serum level of CK-MB, haemodynamic parameters including rate of rise and decline of left ventricular pressure (± dp/dtmax), left ventricular systolic pressure (LVSP), main arterial pressure (MAP), coronary blood flow (CBF), coronary vascular resistance (CVR) were measured in this study. TUNEL positive cells were markedly reduced from 27.83 ± 8.15 % in the control dogs to 7.74 ± 3.50 % in the TFEB-treated ones (P < 0.01), consistent with the absence of DNA laddering. TFEB significantly reduced infarct size from 19.30 ± 6.66 % in the control dogs to 8.87 ± 1.66 % (P < 0.01), confirmed by lower serum CK-MB activity. TFEB significantly reduced MAP and CVR. The decrease in ± dp/dtmax, LVSP and CBF also tended to be much smaller in the TFEB treated group. Conclusion — These data suggest that the inhibitory effect of TFEB on apoptosis during coronary occlusion is associated with reduction in infarct size and improvement in heart function. Objective — The current study tests the hypothesis that inhibition of apoptotic cell death with total flavones from Elsholtzia blanda (TFEB), a traditional Chinese medicine, reduces infarct size and improves heart function during myocardial ischaemia induced by coronary occlusion in canines. Methods and results — Myocardial apoptosis was detected by the TdT-mediated dUTP nick end labelling (TUNEL) method and DNA laddering. Infarct size of the left ventricle, serum level of CK-MB, haemodynamic parameters including rate of rise and decline of left ventricular pressure (± dp/dtmax), left ventricular systolic pressure (LVSP), main arterial pressure (MAP), coronary blood flow (CBF), coronary vascular resistance (CVR) were measured in this study. TUNEL positive cells were markedly reduced from 27.83 ± 8.15 % in the control dogs to 7.74 ± 3.50 % in the TFEB-treated ones (P < 0.01), consistent with the absence of DNA laddering. TFEB significantly reduced infarct size from 19.30 ± 6.66 % in the control dogs to 8.87 ± 1.66 % (P < 0.01), confirmed by lower serum CK-MB activity. TFEB significantly reduced MAP and CVR. The decrease in ± dp/dtmax, LVSP and CBF also tended to be much smaller in the TFEB treated group. Conclusion — These data suggest that the inhibitory effect of TFEB on apoptosis during coronary occlusion is associated with reduction in infarct size and improvement in heart function. |
