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Document Details : Title: Effects of copper and zinc on the production of hoimocysteine-induced extracellular matrix metalloproteinase-2 in cultured rat vascular smooth muscle cells Author(s): GUO, Hangyuan , LEE, Jong-Dae , UZUI, Hiroyasu , TOYODA, Kiyohiro , GESHI, Tooru , YUE, Hong , UEDA, Takanori Journal: Acta Cardiologica Volume: 60 Issue: 4 Date: August 2005 Pages: 353-359 DOI: 10.2143/AC.60.4.2004982 Abstract : Objective — To study the effects of copper and zinc on the production of homocysteine-induced extracellular matrix metalloproteinase-2 in cultured rats vascular smooth muscle cells. Methods — We examined the effects of different homocysteine levels (0-1000 mmol/l) on matrix metalloproteinase-2 (MMP-2) production, and the effects of different copper and zinc concentrations (0-300 mmol/l) on homocysteine-induced MMP-2 in cultured rat vascular smooth muscle cells (VSMCs) using gelatin zymography and western blotting. We further compared the changes of MMP-2 under various treatments for 24, 48 and 72 hours. Results — Homocysteine (50-1000 mmol/l) increased the production of MMP-2 significantly in a dose-dependent manner. Copper decreased the homocysteine-induced MMP-2 under these conditions in a dose-dependent manner, but zinc did not. Production of MMP-2 under various treatments for 72 h increased more than 24 h and 48 h. Conclusions — Extracellular added copper decreased homocysteine-induced MMP-2 secretion. |
